Bioremediation – Projects

Here are a few of our completed Bioremediation projects that we have completed since 1991! Contact us today!
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Bioremediation Projects

BioWorld Bioremediation Treatment uses the most innovative biotechnology to provide a cost-effective and ecological algae treatment for ponds, lakes, canals and more. See below for Bioremediation projects where our treatment was used in bioremediation projects spanning more than 25 years.

Crude Oil Sludge – Drilling Waste
Project #1025

Oil sludge (over 25 years old) from a drilling waste holding pond was provided to BioWorld for bench scale testing to demonstrate the feasibility of BioWorld Bioremediation Technology.

The initial Total Petroleum Hydrocarbon (TPH) concentration of the sludge was 76,000 ppm. Samples designated untreated and treated were placed in separate glass aquariums with the same amount of distilled water in each aquarium.

BioWorld Bioenhancement and Augmentation Technology consisting of Liquid Optimizer and Hydrocarbon Digesting Microbes were added several times to the treated sludge aquarium.

After three months, the water from each aquarium was allowed to evaporate, leaving a solid material behind. Chemical analysis of the untreated and treated sludge samples indicated TPH concentrations of 75,000 ppm and 31,000 ppm, respectively. This corresponds to a 60% reduction in TPH concentration using BioWorld Bioremediation Technology.

Oil Water Separator
Project Summary #564

A tractor repair facility uses a 600-gallon recirculating oil/water separator and steam cleaner system to pressure wash tractors. Oily wastewater determined to be hazardous collects in the separator system. 

BioWorld Liquid Optimizer and Hydrocarbon Digesting Microbes were added to treat the oil. After three days, breakdown and digestion of the petroleum hydrocarbons was apparent. On-going weekly treatments continued to digest the oily waste into non-hazardous substances.

Mixed Transportation Waste
Project #812

A tank transport facility generates wastewater containing mixed vegetable oil and petroleum hydrocarbons. The wastewater collects in a concrete-lined pit. 

BioWorld Liquid Optimizer, Hydrocarbon Digesting Microbes and General Blend Microbes were added several times to treat the mixed waste. Digestion of the mixed waste was apparent and a reduction in TPH and Oil & Grease concentrations was verified.

Clean Up of Contaminated Groundwater
Project #1334

A site in Burlingame, California had groundwater contaminated with free product (gasoline) that required clean up.  An unpleasant odor was observed at the monitoring wells.   

A small quantity of BioWorld Hydrocarbon Degrading Microbes and BioWorld Enhancement solutions were introduced directly into the groundwater monitoring wells to make contact with the free product. The project was reevaluated after 2 months. The odors were no longer present and no free product was detected.  

The site has been signed-off by the San Mateo County Department of Environmental Health Agency.

Oil In Water Wells
Project #1184

A site in Burlingame, California had groundwater contaminated with free product (gasoline) that required clean up.  An unpleasant odor was observed at the monitoring wells.   

A small quantity of BioWorld Hydrocarbon Degrading Microbes and BioWorld Enhancement solutions were introduced directly into the groundwater monitoring wells to make contact with the free product. The project was reevaluated after 2 months. The odors were no longer present and no free product was detected.  

The site has been signed-off by the San Mateo County Department of Environmental Health Agency.

Crude Oil In Irrigation Water
Project #772

 A citrus ranch used well water for irrigating the trees.  The water was pumped into a 2-acre holding pond prior to going through the filtration system.  The aquifer had crude oil seeping into the well area that would end up in the holding pond.

Advanced BioTech were applied to the pond surface as a regular maintenance program and the crude oil was no longer visible.  The filtration back-flush system had no indications of petroleum hydrocarbons.

California, USA

Surface Oil and Algae In Irrigation Pond and System
Project #1162

A ranching operation has several irrigation reservoirs containing surface oil and algae.  The oil and algae cause problems with pump clogging in the irrigation system.

The reservoirs were treated with BioWorld Liquid Optimizer, Algae Competing Microbes and Hydrocarbon Digesting Microbes on a weekly basis for a few months.  The oil and algae were eliminated from the reservoirs allowing the pumps and irrigation system to work properly.

Oil Contamination of Soil at Construction Site
Project #1907

A construction facility contained above ground diesel tanks and waste oil drums.

Over the years, the tanks and drums leaked resulting in soil contamination.  Approximately 500 cubic yards of soil was stockpiled in preparation for bioremediation.  A biotreatability study was conducted to evaluate if the contaminated soil would support the biological processes necessary to degrade petroleum hydrocarbons.

The results verified the potential for successful bioremediation.  Fieldwork is pending to degrade the diesel and oil contaminated soil to acceptable regulatory levels.

Bioremediation of Crude Oil Contaminated Soil
Project #1095

A petroleum processing facility in Colombia treated 200 cubic meters of crude oil contaminated soil with BioWorld Bioremediation Technology.  The initial total petroleum hydrocarbon (TPH) concentration was greater than 60,000 ppm.  

BioWorld Bioremediation Enhancer and Hydrocarbon Digesting Microbes were added to the soil 3 times over a two-month period.  Product cost was low per cubic meter compared to other methods of cleanup.  Within 2 months, the TPH concentration reached less than 10,000 ppm and the project was considered a success.

Service Station Ground Water Bioremediation Treatment
Project # 10081

Project Situation
  • Ground Water contamination of gasoline containing MTBE – Methyl Tert-Butyl Ether and TBA – Tert-Butyl Alcohol.
  • Pump and treat method was chosen as the best available technology for this project
Products/Technology Used
  • Product # 75XXX – BioWorld Bioremediation Enhancer
  • Product # 32XXX – BioWorld Hydrocarbon Digester Microbes

BioWorld teamed up with an Engineering Firm that combined the equipment with the BioWorld Bioremediation Technology.

Results

Sample chain of custody was completely handled by the service station property owner – a major oil company.  Results taken at third week of BioWorld treatment – retention time of 8 hours on a constant flow system.

Influent

MTBE@140 PPB       

TBA@430 PPB       

Outflow sample after Bioremediation in aerated tank:

MTBE@3.9 PPB     

TBA@110 PPB

Outflow sample after addtional treatment unit:

MTBE                              Non-Detected

TBA                                 Non-Detected

NOTE: Prior to Advanced BioTech being applied, an enzyme product was evaluated with a 30% reduction in MTBE and TBA.  The enzyme product was 3 to 4 times the cost of BioWorld Treatments.  The enzyme product required flow to pass through 3 stages of filters that would clog and release the MTBE and TBA.

Bioremediation Projects – Case Studies

Below you will find more examples of bioremediation projects that we have completed over the years in PDF format:
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